Lactate Dehydrogenase Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30578

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lactate Dehydrogenase Primary Antibodies »

Product Specifications

Immunogen

Between 284 and 334

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lactate Dehydrogenase Antibody - BSA Free

Lactate Dehydrogenase Antibody

Immunoprecipitation:Lactate Dehydrogenase AntibodyNBP3-30578] -

Immunoprecipitation:Lactate Dehydrogenase AntibodyNBP3-30578] -Detection of human Lactate Dehydrogenase B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lactate Dehydrogenase B antibody NBP3-30578 (lot NBP3-30578-1) used for IP at 6 µg per reaction. Lactate Dehydrogenase B was also immunoprecipitated by rabbit anti-Lactate Dehydrogenase B antibody BL18400. For blotting immunoprecipitated Lactate Dehydrogenase B, NBP3-30578 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Lactate Dehydrogenase Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lactate Dehydrogenase

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDHA, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) -in the heart, LDH-2 (3H1M) -in the reticuloendothelial system, LDH-3 (2H2M) -in the lungs, LDH-4 (1H3M) -in the kidneys and LDH-5 (4M) -in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.

Alternate Names

EC 1.1.1, EC 1.1.1.27, lactate dehydrogenase B, LDH heart subunit, LDH-B, LDH-H, L-lactate dehydrogenase B chain, Renal carcinoma antigen NY-REN-46, TRG-5

Gene Symbol

LDHB

Additional Lactate Dehydrogenase Products

Product Documents for Lactate Dehydrogenase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Lactate Dehydrogenase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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