Lactate Dehydrogenase C Antibody (9F2) - BSA Free

Novus Biologicals | Catalog # NBP3-26173

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9F2 expressed in HEK293

Format

BSA Free
View all Lactate Dehydrogenase C Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Lactate Dehydrogenase C [UniProt P07864]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lactate Dehydrogenase C Antibody (9F2) - BSA Free

Lactate Dehydrogenase C Antibody (9F2)

Flow Cytometry: Lactate Dehydrogenase C Antibody (9F2) [NBP3-26173] -

Flow Cytometry: Lactate Dehydrogenase C Antibody (9F2) [NBP3-26173] - Overlay Peak curve showing Hela cells stained with Lactate Dehydrogenase C Antibody (9F2) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Lactate Dehydrogenase C Antibody (9F2) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase C

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa) (2). LDHA and LDHB are expressed in most somatic tissues, while expression of LDHC is confined to the germinal epithelium of the testes (3)

Alternate Names

Cancer/testis antigen 32, CT32EC 1.1.1.27, EC 1.1.1, lactate dehydrogenase C, lactate dehydrogenase C4, LDH testis subunit, LDH3MGC111073, LDH-C, LDHX, LDH-X, L-lactate dehydrogenase C chain

Gene Symbol

LDHC

Additional Lactate Dehydrogenase C Products

Product Documents for Lactate Dehydrogenase C Antibody (9F2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Lactate Dehydrogenase C Antibody (9F2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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