Lactoferrin Antibody (1A1) - BSA Free

Novus Biologicals | Catalog # NBP1-40269

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A1

Format

BSA Free
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Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified lactoferrin from human milk.

Specificity

No cross-reactivity with human transferrin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Lactoferrin Antibody (1A1) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Western Blot

1:100 - 1:2000
Application Notes
Recommended pairs for sandwich immunoassay (capture-detection): NB600-1529 - NBP1-40269.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lactoferrin

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. [provided by RefSeq]

Alternate Names

GIG12, HEL110, HLF2, Kaliocin-1, Lactoferricin, Lactoferroxin, Lactotransferrin, LF, LTF, Talalactoferrin

Entrez Gene IDs

4057 (Human)

Gene Symbol

LTF

UniProt

Additional Lactoferrin Products

Product Documents for Lactoferrin Antibody (1A1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lactoferrin Antibody (1A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Lactoferrin Antibody (1A1) - BSA Free

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  • Q: Is required any pre-treatment in microwave oven before to apply the antibody? Is needed a peculiar inhibition for endogenous peroxidase or is it enough to perform the usual treatment with hydrogen peroxide or methanol? Thanks in advance

    A:

    Our Lactoferrin antibody with catalogue number NBP1-40269 is covered by our 100% guarantee for IHC with paraffin-embedded human samples. We recommend the use of heat induced antigen retrieval in pH 6.0 citrate buffer. Please read our protocol for IHC-P, which includes details of the hydrogen peroxide / methanol method of quenching endogenous peroxide activity. The recommended starting dilution when using this antibody for IHC-P is 10ug/ml.

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