Lamin B2 Antibody (144) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43834

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 144

Format

Azide and BSA Free
View all Lamin B2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin B2. The exact sequence is proprietary.

Reactivity Notes

Bovine (84%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lamin B2 Antibody (144) - Azide and BSA Free

Immunohistochemistry-Paraffin: Lamin B2 Antibody (144) [NBP2-43834]

Immunohistochemistry-Paraffin: Lamin B2 Antibody (144) [NBP2-43834]

Immunohistochemistry-Paraffin: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of Cal27 xenograft. Lamin B2 antibody [144] dilution: 1:200.
Western Blot: Lamin B2 Antibody (144) [NBP2-43834]

Western Blot: Lamin B2 Antibody (144) [NBP2-43834]

Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of A. 30 ug HepG2 whole cell lysate/extract B. 30 ug HepG2 cytosol lysate/extract C. 30 ug HepG2 nuclear lysate/extract 7.5 % SDS-PAGE Lamin B2 antibody [144] dilution: 1:1000.
Western Blot: Lamin B2 Antibody (144) [NBP2-43834]

Western Blot: Lamin B2 Antibody (144) [NBP2-43834]

Western Blot: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 7.5 % SDS-PAGE Lamin B2 antibody [144] dilution: 1:1000
Immunoprecipitation: Lamin B2 Antibody (144) [NBP2-43834]

Immunoprecipitation: Lamin B2 Antibody (144) [NBP2-43834]

Immunoprecipitation: Lamin B2 Antibody (144) [NBP2-43834] - Analysis of HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of Lamin B2 by 3 ug of Lamin B2 antibody [144] 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [144] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Lamin B2 Antibody (144) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (144) [NBP2-43834] -

Lamin B2 antibody [GT144] detects LMNB2 protein at nuclear envelope by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min.
Red: LMNB2 protein stained by Lamin B2 antibody [GT144] (NBP2-43834) diluted at 1:1000.
Green: alpha Tubulin antibody diluted at 1:1000.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
Lamin B2 Antibody (144) - Azide and BSA Free

Western Blot: Lamin B2 Antibody (144) [NBP2-43834] -

Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B2 antibody [GT144] (NBP2-43834) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for Lamin B2 Antibody (144) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lamin B2

An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of a-helices and a carboxyterminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organized into loops, which are attached to the nuclear matrix. This organization is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins.

Alternate Names

lamin B2, lamin-B2, LMN2LAMB2, MGC2721

Gene Symbol

LMNB2

Additional Lamin B2 Products

Product Documents for Lamin B2 Antibody (144) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lamin B2 Antibody (144) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lamin B2 Antibody (144) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Lamin B2 Antibody (144) - Azide and BSA Free

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  • Q: I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?

    A:

    We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

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