Lamin B2 Antibody (2E2F4) - BSA Free

Novus Biologicals | Catalog # NBP2-61818

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2E2F4

Format

BSA Free
View all Lamin B2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Lamin B2 (AA: 401-600) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

67.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lamin B2 Antibody (2E2F4) - BSA Free

Western Blot: Lamin B2 Antibody (2E2F4)BSA Free [NBP2-61818]

Western Blot: Lamin B2 Antibody (2E2F4)BSA Free [NBP2-61818]

Western Blot: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis using LMNB2 mAb against HEK293 (1) and LMNB2 (AA: 401-600)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of HepG2 cells using LMNB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunohistochemistry-Paraffin: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunohistochemistry-Paraffin: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of ovarian cancer tissues using LMNB2 mouse mAb with DAB staining.
Flow Cytometry: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Flow Cytometry: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Flow Cytometry: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of Hela cells using LMNB2 mouse mAb (green) and negative control (red).
Western Blot: Lamin B2 Antibody (2E2F4)BSA Free [NBP2-61818]

Western Blot: Lamin B2 Antibody (2E2F4)BSA Free [NBP2-61818]

Western Blot: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis using LMNB2 mAb against human LMNB2 (AA: 401-600) recombinant protein. (Expected MW is 47.6 kDa)
Western Blot: Lamin B2 Antibody (2E2F4)BSA Free [NBP2-61818]

Western Blot: Lamin B2 Antibody (2E2F4)BSA Free [NBP2-61818]

Western Blot: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis using LMNB2 mouse mAb against PC-3 (1), LNcap (2), Jurkat (3), and HCT116 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of GC-7901 cells using LMNB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunocytochemistry/ Immunofluorescence: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunocytochemistry/Immunofluorescence: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of Hela cells using LMNB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunohistochemistry-Paraffin: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

Immunohistochemistry-Paraffin: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of colon cancer tissues using LMNB2 mouse mAb with DAB staining.
ELISA: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

ELISA: Lamin B2 Antibody (2E2F4) - BSA Free [NBP2-61818]

ELISA: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for Lamin B2 Antibody (2E2F4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lamin B2

This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy.;

Alternate Names

lamin B2, lamin-B2, LMN2LAMB2, MGC2721

Gene Symbol

LMNB2

Additional Lamin B2 Products

Product Documents for Lamin B2 Antibody (2E2F4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Lamin B2 Antibody (2E2F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Lamin B2 Antibody (2E2F4) - BSA Free

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  • Q: I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?

    A: We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

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