Laminin alpha 5 Antibody

Novus Biologicals | Catalog # NBP1-18714

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Laminin alpha 5 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide derived from Cter domain of Lama5 protein

Specificity

Reacts specifically with mouse 404 kDa protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Laminin alpha 5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:200 - 1:1000
Application Notes
Use in IHC reported in scientific literature (PMID: 30139844).

Reviewed Applications

Read 1 review rated 1 using NBP1-18714 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in distilled water.

Formulation

Antiserum

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin alpha 5

Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins are thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin alpha 5 (LAMA5) belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Laminin alpha 5 is the alpha chain in the Laminin 15 complex which is a heterotrimer composed of alpha 5/beta 2/gamma 3 which are bound to each other by disulfide bonds into a cross shaped molecule comprising one long and three short arms with globules at each end.

Alternate Names

KIAA0533, KIAA1907, laminin alpha-5 chain, laminin subunit alpha-5, laminin, alpha 5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

Entrez Gene IDs

16776 (Mouse)

Gene Symbol

LAMA5

UniProt

Q61001 (Mouse)

Additional Laminin alpha 5 Products

Product Documents for Laminin alpha 5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Laminin alpha 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Laminin alpha 5 Antibody

Customer Reviews for Laminin alpha 5 Antibody (1)

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  • Laminin alpha 5 Antibody
    Name: Anonymous
    Application: Wholemount Immunofluorescence
    Sample Tested: Mouse Tissues and Mouse tissue
    Species: Mouse
    Verified Customer | Posted 05/03/2021
    Dilution of 1:100 Paired with Secondary: Alexa Fluor Anti-rabbit (Host Goat) 1:200 A-21245 Leica Y5 Filter Exposed for 200 ms..
    Laminin alpha 5 Antibody NBP1-18714
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs for Laminin alpha 5 Antibody

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  • Q: Do you know some papers that are citing this antibody for used in IHC?

    A: Unfortunately we are not currently aware of any publications which cite the use of our laminin alpha 5 antibody, NBP1-18714. This does not mean that such articles do not exist, it is just that we are unaware of them if they do.

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