LAT Antibody

Novus Biologicals | Catalog # NB100-1441

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LAT Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GAPDYENLQELN corresponding to C-Terminus according to NP_055202.1, NP_001014987.1, NP_001014989.1, NP_001014988.1.

Specificity

This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LAT Antibody

Western Blot: LAT Antibody [NB100-1441]

Western Blot: LAT Antibody [NB100-1441]

Western Blot: LAT Antibody [NB100-1441] - (0.01ug/ml) staining of peripheral blood mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for LAT Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in human lymph node and peripheral Brood mononucleocytes lysates and in mouse and rat thymus lysates (calculated MW of 27.9 kDa band according to NP_055202.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LAT

Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD), which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells.

Long Name

Linker for Activation of T cells

Alternate Names

pp36

Entrez Gene IDs

27040 (Human)

Gene Symbol

LAT

UniProt

NP_055202. (Human)

Additional LAT Products

Product Documents for LAT Antibody

Certificate of Analysis

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Product Specific Notices for LAT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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