Legumain/Asparaginyl Endopeptidase Antibody (46I3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-21996

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 46I3

Format

Azide and BSA Free
View all Legumain/Asparaginyl Endopeptidase Primary Antibodies »

Product Specifications

Immunogen

Human recombinant Legumain/Asparaginyl Endopeptidase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (46I3)Azide and BSA Free [NBP2-21996]

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (46I3)Azide and BSA Free [NBP2-21996]

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (46I3) [NBP2-21996] - Lysates of human placental tissue.

Applications

Application
Recommended Usage

ELISA

1:1000-1:2000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Legumain/Asparaginyl Endopeptidase

Legumain encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq]

Alternate Names

AEP, Asparaginyl Endopeptidase, LGMN

Gene Symbol

LGMN

UniProt

Q99538

Additional Legumain/Asparaginyl Endopeptidase Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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