Lewis Y Antibody (LWY/9243R) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-24188
Recombinant Monoclonal Antibody
![Lewis Y Antibody (LWY/9243R) - Azide and BSA Free Immunohistochemistry-Paraffin: Lewis Y Antibody (LWY/9243R) - Azide and BSA Free [NBP3-24188] -](https://resources.rndsystems.com/images/products/nbp3-24188_rabbit-lewis-y-mab-lwy-9243r-azide-and-bsa-free-24120257592896.jpg "Immunohistochemistry-Paraffin: Lewis Y Antibody (LWY/9243R) - Azide and BSA Free [NBP3-24188] -")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Kappa Clone # LWY/9243R
Format
Azide and BSA Free
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Product Specifications
Immunogen
H3396 cultured line established from a metastasis of a human breast adenocarcinoma.
Localization
Cell membrane. Cytoplasm. Extracellular.
Marker
Tumor Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for Lewis Y Antibody (LWY/9243R) - Azide and BSA Free
Immunohistochemistry-Paraffin: Lewis Y Antibody (LWY/9243R) - Azide and BSA Free [NBP3-24188] -
Immunohistochemistry-Paraffin: Lewis Y Antibody (LWY/9243R) - Azide and BSA Free [NBP3-24188] - Formalin-fixed, paraffin-embedded human stomach stained with Lewis Y Antibody (LWY/9243R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Lewis Y Antibody (LWY/9243R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Lewis Y
Alternate Names
IPCA-2, IPCA6, IPCA-6, IPCA-8, PCANAP2, PCANAP6, PCANAP8, Prostate cancer-associated protein 6, PRST, Solute carrier family 45 member 3
Additional Lewis Y Products
Product Documents for Lewis Y Antibody (LWY/9243R) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Lewis Y Antibody (LWY/9243R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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