Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # L1

Format

BSA Free
View all LH beta Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified natural LH beta.

Specificity

High affinity for the beta chain of LH. No cross-reaction with FSH, TSH or HCG.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for LH beta Antibody (L1) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended pair for sandwich immunoassay (capture - detection): NB100-73010 - NB100-73003.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

10mM Tris (pH 7.5) and 0.15M NaCl

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LH beta

LH is a member of the glycoprotein hormone family. Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.

Long Name

Luteinizing Hormone beta Polypeptide

Alternate Names

CGB4, LHB, LSH-B, Lutropin beta

Entrez Gene IDs

3972 (Human)

Gene Symbol

LHB

Additional LH beta Products

Product Documents for LH beta Antibody (L1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LH beta Antibody (L1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for LH beta Antibody (L1) - BSA Free

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  • Q: Is there any cross-reactivity with LH-alpha?

    A: Our LH beta antibody with catalogue number NB100-73010 was raised against purified human LH beta, for which it has a high affinity. It has been shown not to cross-react with FSH, TSH or HCG, however we unfortunately have no testing data where we have used the antibody against LH alpha. An alignment of the human LHB and LHA sequences shows little homology, and so cross-reactivity seems unlikely, however we have not confirmed this in our lab.

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