LHR Antibody - BSA Free

Novus Biologicals | Catalog # NLS1436

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Bovine, Canine

Cited:

Human, Bovine, Canine

Predicted:

Monkey (95%), Primate (100%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LHR Primary Antibodies »

Product Specifications

Immunogen

Synthetic 19 amino acid peptide from C-terminus of human LHR.

Epitope

C-Terminus

Reactivity Notes

Bovine reactivity reported in scientific literature (Martin I et al). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (95%), Bat (89%), Rabbit (89%), Equine (89%). Use in Canine reported in scientific publication (PMID: 32584181).

Localization

Secreted

Specificity

Human LHR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for LHR Antibody - BSA Free

Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - Anti-LHCGR / LHR / LH Receptor antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - Ovary, Graafian Follicle
Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - Placenta
Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - Colon Myenteric Plexus
Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - Anti-LHCGR / LHR / LH Receptor antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody - BSA Free [NLS1436]

Immunohistochemistry-Paraffin: LHR Antibody [NLS1436] - Anti-hCG receptor antibody IHC of human ovary, corpus luteum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Applications for LHR Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml
Application Notes
.

Reviewed Applications

Read 1 review rated 4 using NLS1436 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: LHR

LHCGR has been reported in brain, cervix, epididymis, ovary, placenta, prostate, testis, umbilical cord, and uterus. ESTs have been isolated from fetal lung/testis/B-cell and testis libraries.

Long Name

Luteinizing Hormone Receptor/Choriogonadotropin Receptor

Alternate Names

CGR, HHG, LCGR, LGR2, LHCGR, ULG5

Entrez Gene IDs

3973 (Human)

Gene Symbol

LHCGR

UniProt

P22888 (Human)

Additional LHR Products

Product Documents for LHR Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for LHR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LHR Antibody - BSA Free

Customer Reviews for LHR Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/22/2014
    LHR staining in a middle size antral follicle (1.5 mm) of human ovary
    LHR Antibody - BSA Free NLS1436

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Protocols

View specific protocols for LHR Antibody - BSA Free (NLS1436):

Immunohistochemistry Protocol for Luteinizing Hormone Receptor Antibody (NLS1436):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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FAQs for LHR Antibody - BSA Free

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  • Q: Could you please tell me the exact homology between the sequence used to generate the NLS1436 antibody and the bovine LHR amino acid sequence?

    A: The immunogen, which is proprietary, is 19 amino acids long and shares 17 similar amino acids with the bovine LHR (http://www.uniprot.org/uniprot/Q28005#sequences) sequence (89%).

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