![Western Blot: LIMD1 Antibody [NBP1-71937]](https://resources.rndsystems.com/images/products/LIMD1-Antibody-Western-Blot-NBP1-71937-img0002.jpg "Western Blot: LIMD1 Antibody [NBP1-71937]")
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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 50 and 100 of human LIM Domains Containing 1 using the numbering given in entry NP_055055.1 (GeneID 8994).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LIMD1 Antibody
Western Blot: LIMD1 Antibody [NBP1-71937]
Western Blot: LIMD1 Antibody [NBP1-71937] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-LIMD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. LIMD1 was also immunoprecipitated by rabbit anti-LIMD1 antibody NBP1-71936, which recognizes an upstream epitope.Applications for LIMD1 Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 mcg/mg
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: LIMD1
Long Name
LIM Domains Containing 1
Alternate Names
LIM domain-containing protein 1, LIM domains containing 1
Gene Symbol
LIMD1
UniProt
Additional LIMD1 Products
Product Documents for LIMD1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LIMD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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