Human Liver Whole Tissue Lysate (Adult Whole Normal)
Novus Biologicals | Catalog # NB820-59232
Key Product Details
Species
Applications
Product Specifications
Applications
Application Notes
Specificity
Type
Life Stage
Tissue
Protein State
Tissue Condition
Subcellular Fraction
Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: Liver
Alternate Names
Additional Liver Products
Product Documents for Human Liver Whole Tissue Lysate (Adult Whole Normal)
Certificate of Analysis
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Product Specific Notices for Human Liver Whole Tissue Lysate (Adult Whole Normal)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
Citations for Human Liver Whole Tissue Lysate (Adult Whole Normal)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human Liver Whole Tissue Lysate (Adult Whole Normal)
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Q: Is Human Liver Whole Tissue Lysate (Adult Whole Normal, cat. no. NB820-59232) produced from a single person or a group of people. If it is from a single person, may we ask about the gender and the age of the donor?
A: Donor information is lot specific. Please contact the Technical support team.
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Q: Regarding Human Liver Whole Tissue Lysate (Adult Whole Normal) NB820-59232 and Human Liver Whole Tissue Lysate (Adult Whole Liver Cirrhosis): 1. Does the lysate also contain the membrane protein? If yes, will it be possible to isolate the membrane fraction from the lysate? For my experiment I segregate the cytosolic and membrane fraction. 2. Do you treat, Human Liver Whole Tissue Lysate (Adult Whole Liver Cirrhosis) NB820-59328, with DNase1 to avoid coagulation while heating prior to loading the lysate in SDS running gel? If not DNase1, what you use to avoid the coagulation?
A: All Whole Tissue Lysates are prepared using the following kit: NBP2-37853
We don’t treat the lysates with DNase. We do not notice any coagulation when loading on an SDS-PAGE gel. Just heating the sample in standard loading buffer works well.
The whole tissue lysates should contain plasma membrane fragments, however some components (e.g. mitochondria, lysosomes) will probably precipitate in the final 18,000g spin.
We do however have corresponding membrane fractions:
Normal: NB820-60598
Normal, left lobe: NB820-60599
Cirrhosis: NBL1-26449 -
Q: Which fraction is contained in this lysate? Also, on the datasheet, the applications of this lysate include enzyme activity analysis - what does this mean? Lastly, could you tell me if using RIPA buffer affects the enzyme activity or not?
A: The adult normal human liver whole tissue lysate with catalog number NB820-59232 is prepared from whole tissue homogenates and is stored in HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent with a cocktail of protease inhibitors. As such, the activity of the expressed enzymes should be preserved. During the Quality Control process the protein lysate is verified on SDS-PAGE analysis as a consistent pattern comparing to a standard protein lysate. This uses the following biomarkers: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein. NB820-59232 is validated for Western blotting and immunoprecipitation, and is guaranteed for 6 months from the date of receipt. We recommend that you store this product at -80C and avoid freeze-thaw cycles.
-
Q: Is Human Liver Whole Tissue Lysate (Adult Whole Normal, cat. no. NB820-59232) produced from a single person or a group of people. If it is from a single person, may we ask about the gender and the age of the donor?
A: Donor information is lot specific. Please contact the Technical support team.
-
Q: Regarding Human Liver Whole Tissue Lysate (Adult Whole Normal) NB820-59232 and Human Liver Whole Tissue Lysate (Adult Whole Liver Cirrhosis): 1. Does the lysate also contain the membrane protein? If yes, will it be possible to isolate the membrane fraction from the lysate? For my experiment I segregate the cytosolic and membrane fraction. 2. Do you treat, Human Liver Whole Tissue Lysate (Adult Whole Liver Cirrhosis) NB820-59328, with DNase1 to avoid coagulation while heating prior to loading the lysate in SDS running gel? If not DNase1, what you use to avoid the coagulation?
A: All Whole Tissue Lysates are prepared using the following kit: NBP2-37853
We don’t treat the lysates with DNase. We do not notice any coagulation when loading on an SDS-PAGE gel. Just heating the sample in standard loading buffer works well.
The whole tissue lysates should contain plasma membrane fragments, however some components (e.g. mitochondria, lysosomes) will probably precipitate in the final 18,000g spin.
We do however have corresponding membrane fractions:
Normal: NB820-60598
Normal, left lobe: NB820-60599
Cirrhosis: NBL1-26449 -
Q: Which fraction is contained in this lysate? Also, on the datasheet, the applications of this lysate include enzyme activity analysis - what does this mean? Lastly, could you tell me if using RIPA buffer affects the enzyme activity or not?
A: The adult normal human liver whole tissue lysate with catalog number NB820-59232 is prepared from whole tissue homogenates and is stored in HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent with a cocktail of protease inhibitors. As such, the activity of the expressed enzymes should be preserved. During the Quality Control process the protein lysate is verified on SDS-PAGE analysis as a consistent pattern comparing to a standard protein lysate. This uses the following biomarkers: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein. NB820-59232 is validated for Western blotting and immunoprecipitation, and is guaranteed for 6 months from the date of receipt. We recommend that you store this product at -80C and avoid freeze-thaw cycles.
-
Q: Is Human Liver Whole Tissue Lysate (Adult Whole Normal, cat. no. NB820-59232) produced from a single person or a group of people. If it is from a single person, may we ask about the gender and the age of the donor?
A: Donor information is lot specific. Please contact the Technical support team.
-
Q: Regarding Human Liver Whole Tissue Lysate (Adult Whole Normal) NB820-59232 and Human Liver Whole Tissue Lysate (Adult Whole Liver Cirrhosis): 1. Does the lysate also contain the membrane protein? If yes, will it be possible to isolate the membrane fraction from the lysate? For my experiment I segregate the cytosolic and membrane fraction. 2. Do you treat, Human Liver Whole Tissue Lysate (Adult Whole Liver Cirrhosis) NB820-59328, with DNase1 to avoid coagulation while heating prior to loading the lysate in SDS running gel? If not DNase1, what you use to avoid the coagulation?
A: All Whole Tissue Lysates are prepared using the following kit: NBP2-37853
We don’t treat the lysates with DNase. We do not notice any coagulation when loading on an SDS-PAGE gel. Just heating the sample in standard loading buffer works well.
The whole tissue lysates should contain plasma membrane fragments, however some components (e.g. mitochondria, lysosomes) will probably precipitate in the final 18,000g spin.
We do however have corresponding membrane fractions:
Normal: NB820-60598
Normal, left lobe: NB820-60599
Cirrhosis: NBL1-26449 -
Q: Which fraction is contained in this lysate? Also, on the datasheet, the applications of this lysate include enzyme activity analysis - what does this mean? Lastly, could you tell me if using RIPA buffer affects the enzyme activity or not?
A: The adult normal human liver whole tissue lysate with catalog number NB820-59232 is prepared from whole tissue homogenates and is stored in HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent with a cocktail of protease inhibitors. As such, the activity of the expressed enzymes should be preserved. During the Quality Control process the protein lysate is verified on SDS-PAGE analysis as a consistent pattern comparing to a standard protein lysate. This uses the following biomarkers: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein. NB820-59232 is validated for Western blotting and immunoprecipitation, and is guaranteed for 6 months from the date of receipt. We recommend that you store this product at -80C and avoid freeze-thaw cycles.