Livin Antibody

Novus Biologicals | Catalog # NB300-941

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Peptide ELISA, Flow (Intracellular)

Cited:

Flow Cytometry Control

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Livin Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RAPVRSRVRT corresponding to C-Terminus according to NP_647478.1, NP_071444.1.

Specificity

This antibody is expected to recognise both reported isoforms (as represented by NP_647478.1 and NP_071444.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Livin Antibody

Western Blot: Livin Antibody [NB300-941]

Western Blot: Livin Antibody [NB300-941]

Western Blot: Livin Antibody [NB300-941] - (0.3ug/ml) of MOLT4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for Livin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 30 kDa band and 35 kDa band in lysates of Molt4, and 30 kDa band in lysates of Daudi, Jurkat and K562. These bands correspond to the reported isoforms with calculated MW of 32.8 kDa band according to NP_647478.1 and 30.9 kDa band according to NP_071444.1. Use in Flow-intracellular reported in scientific literature (PMID: 20068175).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Livin

The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide.

Alternate Names

BIRC7, KIAP, ML-IAP

Entrez Gene IDs

79444 (Human)

Gene Symbol

BIRC7

UniProt

NP_647478 (Human)

Additional Livin Products

Product Documents for Livin Antibody

Certificate of Analysis

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Product Specific Notices for Livin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail