LPS-induced CXC chemokine (LIX) is a member of the murine chemokine family. It is 132 amino acids (aa) in length with a predicted molecular weight of 14 kDa prior to cleavage of the N-terminal signal peptide (aa 1–40). LIX has no true human ortholog but is closely related to human CXCL5/ENA-78 and CXCL6/GCP-2. It is widely expressed, and is secreted by multiple cell types including fibroblasts, thymic epithelium, platelets, vascular endothelium, and hepatocytes. LIX acts as a proinflammatory chemokine and is chemotactic for neutrophils.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Neutralization
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse LIX
Val45-Ala118
Accession # P50228
Val45-Ala118
Accession # P50228
Specificity
Detects mouse LIX in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human GCP‑2, recombinant rat (rr) CINC‑2 alpha, rrCINC-2 beta, recombinant mouse (rm) KC, and rmMIP‑2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for LIX Antibody [FITC]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: LIX
Alternate Names
LIX
Gene Symbol
CXCL5
Additional LIX Products
Product Documents for LIX Antibody [FITC]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LIX Antibody [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Western Blot Figures
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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