LRRC46 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-62692
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LDLSENLIETLKLDEFPQSLLILNLSGNSCTNQDGYRELVTEALPLLLDLDGQPVVERWISDEEDEASSDEEFPELSGPFCSERGFLKELEQE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for LRRC46 Antibody - BSA Free
Immunohistochemistry-Paraffin: LRRC46 Antibody [NBP2-62692]
Immunohistochemistry-Paraffin: LRRC46 Antibody [NBP2-62692] - Staining of human fallopian tube shows high expression.Immunohistochemistry-Paraffin: LRRC46 Antibody [NBP2-62692]
Immunohistochemistry-Paraffin: LRRC46 Antibody [NBP2-62692] - Staining of human pancreas shows low expression as expected.Immunohistochemistry: LRRC46 Antibody - BSA Free [NBP2-62692] -
Spermatogenesis defects of Lrrc46 knockout mice. (a) HE-staining of testes sections from the Lrrc46+/+ and Lrrc46−/− male mice. (b) IF of anti-alpha / beta -tubulin (red) antibodies in testes sections from the Lrrc46+/+ and Lrrc46−/− male mice. (c) The PAS and HE-staining analysis of the testis seminiferous tubule cross-sections of Lrrc46+/+ and Lrrc46−/− male mice. The arrows highlight germ cells at various stages of spermatogenesis. Defects in the nuclear shape of several elongating spermatids were clearly evident in the Lrrc46−/− male mice seminiferous tubule (red circle). P: pachytene spermatocyte, D: diploneme spermatocyte, Z: zygotene spermatocyte, M: meiotic spermatocyte, rST: round spermatid, eST: elongating spermatid, spz: spermatozoa. (d) The PAS and HE-staining analysis of spermatids at different steps from Lrrc46+/+ and Lrrc46−/− male mice. During step 1 to step 10 spermatids of acrosome development period, the sperm acrosome morphology was roughly normal in the Lrrc46−/− male mice. During step 11 to step 18, te spermatids head shaping period, the sperm had an abnormal, club-shaped sperm head morphology in Lrrc46−/− male mice while Lrrc46+/+ mice had normal, hook shaped heads. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35955660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: LRRC46 Antibody - BSA Free [NBP2-62692] -
Spermatogenesis defects of Lrrc46 knockout mice. (a) HE-staining of testes sections from the Lrrc46+/+ and Lrrc46−/− male mice. (b) IF of anti-alpha / beta -tubulin (red) antibodies in testes sections from the Lrrc46+/+ and Lrrc46−/− male mice. (c) The PAS and HE-staining analysis of the testis seminiferous tubule cross-sections of Lrrc46+/+ and Lrrc46−/− male mice. The arrows highlight germ cells at various stages of spermatogenesis. Defects in the nuclear shape of several elongating spermatids were clearly evident in the Lrrc46−/− male mice seminiferous tubule (red circle). P: pachytene spermatocyte, D: diploneme spermatocyte, Z: zygotene spermatocyte, M: meiotic spermatocyte, rST: round spermatid, eST: elongating spermatid, spz: spermatozoa. (d) The PAS and HE-staining analysis of spermatids at different steps from Lrrc46+/+ and Lrrc46−/− male mice. During step 1 to step 10 spermatids of acrosome development period, the sperm acrosome morphology was roughly normal in the Lrrc46−/− male mice. During step 11 to step 18, te spermatids head shaping period, the sperm had an abnormal, club-shaped sperm head morphology in Lrrc46−/− male mice while Lrrc46+/+ mice had normal, hook shaped heads. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35955660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunohistochemistry: LRRC46 Antibody - BSA Free [NBP2-62692] -
Spermatogenesis defects of Lrrc46 knockout mice. (a) HE-staining of testes sections from the Lrrc46+/+ and Lrrc46−/− male mice. (b) IF of anti-alpha / beta -tubulin (red) antibodies in testes sections from the Lrrc46+/+ and Lrrc46−/− male mice. (c) The PAS and HE-staining analysis of the testis seminiferous tubule cross-sections of Lrrc46+/+ and Lrrc46−/− male mice. The arrows highlight germ cells at various stages of spermatogenesis. Defects in the nuclear shape of several elongating spermatids were clearly evident in the Lrrc46−/− male mice seminiferous tubule (red circle). P: pachytene spermatocyte, D: diploneme spermatocyte, Z: zygotene spermatocyte, M: meiotic spermatocyte, rST: round spermatid, eST: elongating spermatid, spz: spermatozoa. (d) The PAS and HE-staining analysis of spermatids at different steps from Lrrc46+/+ and Lrrc46−/− male mice. During step 1 to step 10 spermatids of acrosome development period, the sperm acrosome morphology was roughly normal in the Lrrc46−/− male mice. During step 11 to step 18, te spermatids head shaping period, the sperm had an abnormal, club-shaped sperm head morphology in Lrrc46−/− male mice while Lrrc46+/+ mice had normal, hook shaped heads. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35955660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for LRRC46 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRRC46
Alternate Names
FLJ23553, leucine rich repeat containing 46, leucine-rich repeat-containing protein 46, MGC16309
Gene Symbol
LRRC46
Additional LRRC46 Products
Product Documents for LRRC46 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LRRC46 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for LRRC46 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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