LRRK2 [p Ser935] Antibody (4J10Z7)

Novus Biologicals | Catalog # NBP3-16928

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4J10Z7 expressed in HEK293
View all LRRK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S935 of human LRRK2 (Q5S007). SNSLG

Modification

p Ser 935

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRRK2 [p Ser935] Antibody (4J10Z7)

Western Blot: LRRK2 [p Ser935] Antibody (4J10Z7) [NBP3-16928] - Western blot analysis of extracts of NIH/3T3 cells, using LRRK2 Rabbit mAb (NBP3-16928) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.
Western Blot: LRRK2 [p Ser935] Antibody (4J10Z7) [NBP3-16928] - Western blot analysis of extracts of C6 cells, using LRRK2 Rabbit mAb (NBP3-16928) at 1:1000 dilution.C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for LRRK2 [p Ser935] Antibody (4J10Z7)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LRRK2

LRRK2 is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

LRRK2 antibodies are useful tools for neurodegeneration research and certian kinase signaling pathway studies.

Long Name

Leucine-rich Repeat Ser/Thr Protein Kinase 2

Alternate Names

AURA17, Dardarin, PARK8, ROCO2

Gene Symbol

LRRK2

Additional LRRK2 Products

Product Documents for LRRK2 [p Ser935] Antibody (4J10Z7)

Certificate of Analysis

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Product Specific Notices for LRRK2 [p Ser935] Antibody (4J10Z7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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