LRRK2 Antibody [Alexa Fluor® 647]

Novus Biologicals | Catalog # NB300-268AF647

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, C. elegans, Insect, Plant

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Rabbit IgG
View all LRRK2 Primary Antibodies »

Product Specifications

Immunogen

A C-terminal synthetic peptide made to the human LRRK2 protein sequence (between residues 2500-2527). [UniProt# Q5S007]

Reactivity Notes

Human, Moth reactivity reported in scientific literature (PMID: 19824698). Plant reactivity reported in scientific literature (PMID: 27273569).

Localization

Mitochondrial outer membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LRRK2 Antibody [Alexa Fluor® 647]

Flow Cytometry: LRRK2 Antibody [Alexa Fluor® 647] [NB300-268AF647] - An intracellular stain was performed on U2OS cells with LRRK Antibody NB300-268AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: LRRK2 Antibody [Alexa Fluor (R) 647] [NB300-268AF647] - analysis of LRRK2 in normal human peripheral blood cells (anticoagulated Lithium-Heparin) using anti-LRRK2 antibody conjugated with AF647. The primary antibody was used at a dilution of 1:200 and incubated for 20 min at room temperature. Image from verified customer review.
LRRK2 Antibody [Alexa Fluor® 647]

LRRK2 in U-2 OS Human Cell Line.

LRRK2 was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-LRRK2 Antigen Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB300-268AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for LRRK2 Antibody [Alexa Fluor® 647]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB300-268AF647 in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: LRRK2

LRRK2 is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.

LRRK2 antibodies are useful tools for neurodegeneration research and certian kinase signaling pathway studies.

Long Name

Leucine-rich Repeat Ser/Thr Protein Kinase 2

Alternate Names

AURA17, Dardarin, PARK8, ROCO2

Gene Symbol

LRRK2

Additional LRRK2 Products

Product Documents for LRRK2 Antibody [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LRRK2 Antibody [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LRRK2 Antibody [Alexa Fluor® 647]

Customer Reviews for LRRK2 Antibody [Alexa Fluor® 647] (1)

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Customer Images

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  • Name: Corinna Bliederhaeuser
    Application: Flow Cytometry
    Sample Tested: normal human peripheral blood cells (anticoagulated Lithium-Heparin)
    Species: Human
    Verified Customer | Posted 01/14/2015
    Staining of normal human peripheral blood cells (within the lymphocyte gate) with anti-human HLA-DR Pe-Cy7 and anti-LRRK2 AF647
    LRRK2 Antibody [Alexa Fluor® 647] NB300-268AF647

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