Ly-6C Antibody (ER-MP20) [Biotin]

Novus Biologicals | Catalog # NB100-64203

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # ER-MP20
View all Ly-6C Primary Antibodies »

Product Specifications

Immunogen

Balb/c macrophage precursor cell hybrids

Specificity

Ly6C (ER-MP20)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Ly-6C Antibody (ER-MP20) [Biotin]

Ly-6C Antibody (ER-MP20) [Biotin] Immunohistochemistry: Ly-6C Antibody (ER-MP20) [Biotin] - [NB100-64203] -

Immunohistochemistry: Ly-6C Antibody (ER-MP20) [Biotin] - [NB100-64203] -

Immunohistochemistry: Ly-6C Antibody (ER-MP20) [Biotin] - [NB100-64203] - Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Ly-6C antibody, clone ER-MP20 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody. Low power

Applications for Ly-6C Antibody (ER-MP20) [Biotin]

Application
Recommended Usage

Flow Cytometry

1:50
Application Notes
This antibody is useful for ELISA, Functional, Flow cytometry / FACS analysis, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1% Bovine Serum Albumin

Preservative

0.09% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ly-6C

Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes. This antibody is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by Flow Cytometry. ab15674 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 is recommended. This antibody also detects a wide range of endothelial cells.

Alternate Names

Ly 6c, Ly6c protein, Lymphocyte antigen 6 complex, Lymphocyte antigen 6 complex locus C, Lymphocyte antigen 6C, Lymphocyte antigen Ly 6C

Entrez Gene IDs

17067 (Mouse)

Gene Symbol

Ly6c1

UniProt

P0CW02 (Mouse), P0CW03 (Mouse)

Additional Ly-6C Products

Product Documents for Ly-6C Antibody (ER-MP20) [Biotin]

Certificate of Analysis

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Product Specific Notices for Ly-6C Antibody (ER-MP20) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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