Ly-6C Antibody (ER-MP20) - BSA Free
Novus Biologicals | Catalog # NB100-65413
![Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]](https://resources.rndsystems.com/images/products/LyC6-Antibody-ER-MP20-Immunohistochemistry-NB100-65413-img0007.jpg "Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]")
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # ER-MP20
Format
BSA Free
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Product Specifications
Immunogen
Balb/c macrophage precursor cell hybrids
Specificity
Ly6C (ER-MP20)
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Ly-6C Antibody (ER-MP20) - BSA Free
Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]
Immunohistochemistry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Staining of a mouse lymph node cryosection.![Flow Cytometry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]](https://resources.rndsystems.com/images/products/LyC6-Antibody-ER-MP20-Flow-Cytometry-NB100-65413-img0006.jpg "Flow Cytometry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]")
Flow Cytometry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]
Flow Cytometry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Peripheral Mouse Granulocytes stained with Rat anti Mouse Ly-6C:RPE.![Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]](https://resources.rndsystems.com/images/products/LyC6-Antibody-ER-MP20-Immunohistochemistry-NB100-65413-img0004.jpg "Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]")
Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]
Immunohistochemistry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Staining of a mouse lymph node cryosection.![Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]](https://resources.rndsystems.com/images/products/LyC6-Antibody-ER-MP20-Immunohistochemistry-NB100-65413-img0005.jpg "Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]")
Immunohistochemistry: Ly-6C Antibody (ER-MP20) - BSA Free [NB100-65413]
Immunohistochemistry: Ly-6C Antibody (ER-MP20) [NB100-65413] - Staining of a mouse lymph node cryosection.Applications for Ly-6C Antibody (ER-MP20) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:50
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ly-6C
Alternate Names
Ly 6c, Ly6c protein, Lymphocyte antigen 6 complex, Lymphocyte antigen 6 complex locus C, Lymphocyte antigen 6C, Lymphocyte antigen Ly 6C
Entrez Gene IDs
17067 (Mouse)
Gene Symbol
Ly6c1
UniProt
Additional Ly-6C Products
Product Documents for Ly-6C Antibody (ER-MP20) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Ly-6C Antibody (ER-MP20) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Ly-6C Antibody (ER-MP20) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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