![Immunocytochemistry/ Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989]](https://resources.rndsystems.com/images/products/Lyp-PTPN22-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69989-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence CPPNKPAESVQSNNS, from the internal region of the protein sequence according to NP_057051; NP_036543.4; NP_001180360.1; NP_001295226.1.
Reactivity Notes
Expected from sequence similarity: Chimpanzee
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Lyp/PTPN22 Antibody
Immunocytochemistry/ Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989]
Immunocytochemistry/Immunofluorescence: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989]](https://resources.rndsystems.com/images/products/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0005.jpg "Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989]")
Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989]
Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Flow Cytometry: Lyp/PTPN22 Antibody [NBP2-69989]](https://resources.rndsystems.com/images/products/Lyp-PTPN22-Antibody-Flow-Cytometry-NBP2-69989-img0002.jpg "Flow Cytometry: Lyp/PTPN22 Antibody [NBP2-69989]")
Flow Cytometry: Lyp/PTPN22 Antibody [NBP2-69989]
Flow Cytometry: Lyp/PTPN22 Antibody [NBP2-69989] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989]](https://resources.rndsystems.com/images/products/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0003.jpg "Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989]")
Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989]
Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989]](https://resources.rndsystems.com/images/products/Lyp-PTPN22-Antibody-Immunohistochemistry-Paraffin-NBP2-69989-img0004.jpg "Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989]")
Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989]
Immunohistochemistry-Paraffin: Lyp/PTPN22 Antibody [NBP2-69989] - (3.75ug/ml) Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Lyp/PTPN22 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
3.75 ug/ml
Immunohistochemistry-Paraffin
3.75 ug/ml
Peptide ELISA
1:32000
Western Blot
0.5-1ug/ml
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
TBS (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Lyp
Long Name
Lymphoid Phosphatase
Alternate Names
LyPTP, PEP, PTPN22, PTPN8 (former)
Gene Symbol
PTPN22
Additional Lyp Products
Product Documents for Lyp/PTPN22 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Lyp/PTPN22 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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