Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG3 Clone # E1
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Product Specifications

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.

Reactivity Notes

M13 phage

Specificity

This antibody is specific for the M13 bacteriophage protein coat, amongst others the g3p protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Applications for M13 bacteriophage Antibody (E1) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined..

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: M13 bacteriophage

Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage.May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Alternate Names

Coat protein A; Coat protein B; Coat protein C polypeptide I; Coat protein C polypeptide II; Coat protein D; G3P; III; IX; M13 coat protein A; M13 coat protein B; M13 coat protein C polypeptide I; M13 coat protein C polypeptide II; M13 coat protein D; M13

Additional M13 bacteriophage Products

Product Documents for M13 bacteriophage Antibody (E1) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for M13 bacteriophage Antibody (E1) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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