MACC1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-89352
![Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]](https://resources.rndsystems.com/images/products/MACC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89352-img0011.jpg "Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]")
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FRSGRIAQSMSEANLIDMEAGKLSKSCNITECQDPDLLHNWPDAFTLRGNNASKVANPFWNQLSASNPF
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 25785098).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MACC1 Antibody - BSA Free
Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]
Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352] - Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.![Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]](https://resources.rndsystems.com/images/products/MACC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89352-img0009.jpg "Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]")
Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]
Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352] - Immunohistochemical staining of human colorectal cancer shows moderate to strong cytoplasmic positivity in tumor cells.![Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]](https://resources.rndsystems.com/images/products/MACC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89352-img0010.jpg "Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]")
Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]
Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352] - Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in renal tubules cells.![Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]](https://resources.rndsystems.com/images/products/MACC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89352-img0012.jpg "Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]")
Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352]
Immunohistochemistry-Paraffin: MACC1 Antibody [NBP1-89352] - Immunohistochemical staining of human stomach cancer shows moderate cytoplasmic positivity in tumor cells.![Simple Western: MACC1 Antibody [NBP1-89352]](https://resources.rndsystems.com/images/products/MACC1-Antibody-Simple-Western-NBP1-89352-img0005.jpg "Simple Western: MACC1 Antibody [NBP1-89352]")
Simple Western: MACC1 Antibody [NBP1-89352]
Simple Western: MACC1 Antibody [NBP1-89352] - Simple Western lane view shows a specific band for MACC1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: MACC1 Antibody [NBP1-89352]](https://resources.rndsystems.com/images/products/MACC1-Antibody-Simple-Western-NBP1-89352-img0007.jpg "Simple Western: MACC1 Antibody [NBP1-89352]")
Simple Western: MACC1 Antibody [NBP1-89352]
Simple Western: MACC1 Antibody [NBP1-89352] - Electropherogram image(s) of corresponding Simple Western lane view. MACC1 antibody was used at 1:20 dilution on RT-4 lysate(s).Applications for MACC1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Simple Western
1:20
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, separated by Size, antibody dilution of 1:20, apparent MW was 93 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, separated by Size, antibody dilution of 1:20, apparent MW was 93 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MACC1
Alternate Names
metastasis associated in colon cancer 1, SH3 domain-containing protein 7a5,7A5
Gene Symbol
MACC1
Additional MACC1 Products
Product Documents for MACC1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MACC1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for MACC1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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