MAdCAM-1 Antibody (12B5) - BSA Free

Novus Biologicals | Catalog # NBP3-26157

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 12B5 expressed in HEK293

Format

BSA Free
View all MAdCAM-1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human MAdCAM-1 [UniProt Q13477]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAdCAM-1 Antibody (12B5) - BSA Free

MAdCAM-1 Antibody (12B5)

Flow Cytometry: MAdCAM-1 Antibody (12B5) [NBP3-26157] -

Flow Cytometry: MAdCAM-1 Antibody (12B5) [NBP3-26157] - Overlay Peak curve showing PC3 cells stained with MAdCAM-1 Antibody (12B5) (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for MAdCAM-1 Antibody (12B5) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: MAdCAM-1

Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer's patches and mesenteric lymph nodes, and on flat walled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen. (1-3) The countereceptor or "homing receptor" for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin. (4, 5) The monoclonal antibody MECA-367 binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer's patch HEV in vivo. (1, 2, 4)

Long Name

Mucosal Addressin Cell Adhesion Molecule 1

Alternate Names

MAdCAM1

Gene Symbol

MADCAM1

Additional MAdCAM-1 Products

Product Documents for MAdCAM-1 Antibody (12B5) - BSA Free

Certificate of Analysis

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Product Specific Notices for MAdCAM-1 Antibody (12B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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