MAG/Siglec-4a Antibody (8Q7K5)

Novus Biologicals | Catalog # NBP3-16859

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8Q7K5 expressed in HEK293
View all MAG/Siglec-4a Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAG/Siglec-4a (P20916). MIFLTALPLFWIMISASRGGHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAG/Siglec-4a Antibody (8Q7K5)

Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859]

Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859]

Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859] - Analysis of extracts of SH-SY5Y cells, using MAG/Siglec-4a antibody (NBP3-16859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859]

Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859]

Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859] - Analysis of extracts of Mouse brain cells, using MAG/Siglec-4a antibody (NBP3-16859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859]

Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859]

Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859] - Analysis of extracts of Rat brain cells, using MAG/Siglec-4a antibody (NBP3-16859) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for MAG/Siglec-4a Antibody (8Q7K5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAG/Siglec-4a

Myelin-associated glycoprotein (MAG), a membrane glycoprotein of 100 kDa, is thought to be involved in the process of myelination (1). Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG (2). MAG has been implicated in inhibition of nerve regeneration in the CNS. This results from interactions between MAG and the Nogo receptor and gangliosides on the apposing axon, which generates intracellular inhibitory signals in the neuron (3). Results also suggest that MAG binds to a specific receptor and initiates a signal transduction cascade to effect inhibition. These results indicate that soluble dMAG detected in vivo could contribute to the lack of regeneration in the mammalian CNS after injury (4).

Long Name

Myelin-associated Glycoprotein

Alternate Names

Siglec-4a, Siglec4a

Gene Symbol

MAG

Additional MAG/Siglec-4a Products

Product Documents for MAG/Siglec-4a Antibody (8Q7K5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MAG/Siglec-4a Antibody (8Q7K5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

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