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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DDDDSGWELSMPEKMEKSNTNWVDITQDFEEACRELKLGELLHDKLFGLFEAMSAIEMMDPKMDAGMIGNQVNRKVL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MAK10 Antibody - BSA Free
Western Blot: MAK10 Antibody [NBP2-58424]
Western Blot: MAK10 Antibody [NBP2-58424] - Analysis in human cell line RT-4 and human cell line U-251 MG.Immunocytochemistry/ Immunofluorescence: MAK10 Antibody [NBP2-58424]
Immunocytochemistry/Immunofluorescence: MAK10 Antibody [NBP2-58424] - Paraformaldehyde (4%) fixed HeLa cells were stained with anti-Mak10 antibody and Alexa Fluor 488 anti-rabbit secondary antibody. ICC/IF image submitted by a verified customer review.Immunocytochemistry/ Immunofluorescence: MAK10 Antibody [NBP2-58424]
Immunocytochemistry/Immunofluorescence: MAK10 Antibody [NBP2-58424] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green. Antibody staining is shown in green.Applications for MAK10 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Reviewed Applications
Read 2 reviews rated 5 using NBP2-58424 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MAK10
Alternate Names
bA379P1.1, corneal wound healing-related protein, EGAP, embryonic growth-associated protein homolog, FLJ21613, FLJ22643, MAK10, MAK10 homolog, amino-acid N-acetyltransferase subunit, N(alpha)-acetyltransferase 35, NatC auxiliary subunit, N-alpha-acetyltransferase 35, NatC auxiliary subunit, protein MAK10 homolog
Gene Symbol
NAA35
Additional MAK10 Products
Product Documents for MAK10 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MAK10 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for MAK10 Antibody - BSA Free (2)
5 out of 5
2 Customer Ratings
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Application: ImmunocytochemistrySample Tested: hela cellSpecies: HumanVerified Customer | Posted 12/30/20194% paraformaldehyde fixed HeLa cells were stained with anti-Mak10 antibody and alexa488 anti-rabbit secondary antibody.
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Application: Western BlotSample Tested: IMR-90 human lung fibroblast cell lineSpecies: HumanVerified Customer | Posted 12/30/2019IMR90 whole cell lysate was run on 10% SDS-PAGE.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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