Maltose Binding Protein Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-97370

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Maltose Binding Protein Primary Antibodies »

Product Specifications

Immunogen

This Maltose Binding Protein Antibody was purified from whole rabbit serum prepared by repeated immunizations with the Maltose Binding Protein epitope tag recombinant protein.

Specificity

This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag. To date this antibody has reacted with all MBP tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This IgG purified antibody is directed against Maltose Binding Protein and is useful in determining its presence in various assays

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Maltose Binding Protein Antibody - BSA Free

Western Blot: Maltose Binding Protein AntibodyBSA Free [NBP1-97370]

Western Blot: Maltose Binding Protein AntibodyBSA Free [NBP1-97370]

Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot of Maltose Binding protein antibody - BSA Free.Lane 1: 1.0 ug of recombinant protein containing the Maltose Binding Protein.Primary Antibody: Polyclonal rabbit-anti-Maltose Binding Protein at 0.5-1.0 ug/ml for 1 h at room temperature.Secondary Antibody: 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature. Imaging: LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Predicted MW: ~42 kDa. A minor band at corresponding to multimers of this protein is also evident.
Western Blot: Maltose Binding Protein AntibodyBSA Free [NBP1-97370]

Western Blot: Maltose Binding Protein AntibodyBSA Free [NBP1-97370]

Western Blot: Maltose Binding Protein Antibody - BSA Free [NBP1-97370] - Western Blot showing detection of Maltose Binding protein antibody - BSA Free.Lane 1: MW markers. Lane 2: Maltose Binding Protein [0.05 ug]. Protein was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. Blocking with 1% BSA-TTBS 30 min at 20C. Primary Antibody: Anti-MBP (RABBIT) antibody was used at 1:1000 overnight at 4C. Secondary Antibody: Anti-Rabbit IgG (GOAT) IRDye800(R) conjugated antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at 20C. Imaged on the LiCor Odyssey imaging system. Predicted MW: ~42 kDa, the other bands present are recombinant MBP breakdown.
N/A: Maltose Binding Protein Antibody - BSA Free [NBP1-97370]
Maltose Binding Protein Antibody - BSA Free

Maltose Binding Protein Antibody - BSA Free

Western Blot showing detection of Maltose Binding Protein (MBP). Lane 1: MW markers. Lane 2: Maltose Binding Protein
Maltose Binding Protein Antibody - BSA Free

Maltose Binding Protein Antibody - BSA Free

Anti-MBP epitope tag polyclonal antibody detects MBP-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-MBP epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag. The apparent molecular weight of this band is 42 kDa. A minor band at corresponding to multimers of this protein is also evident. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code ) for 30 min at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Applications for Maltose Binding Protein Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000-1:50000

Western Blot

1:1000-1:5000
Application Notes
This product is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti-MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. This product is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP.

Formulation, Preparation, and Storage

Purification

IgG purified

Reconstitution

For the 1mg size, we recommend reconstitution with 1.0 ml of deionized water. The 0.5mg size is provided in liquid format (0.5 ml)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Maltose Binding Protein

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.

Alternate Names

Mal E, MalE, Maltodextrin binding protein, Maltose ABC transporter periplasmic protein, Maltose binding periplasmic protein, MBP, MMBP, Periplasmic maltose binding protein

Additional Maltose Binding Protein Products

Product Documents for Maltose Binding Protein Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Maltose Binding Protein Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Maltose Binding Protein Antibody - BSA Free

Customer Reviews for Maltose Binding Protein Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Maltose Binding Protein Antibody - BSA Free and earn rewards!

Have you used Maltose Binding Protein Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies