MARCKS [p Ser152, p Ser156] Antibody - Azide Free

Novus Biologicals | Catalog # NB500-140

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat, Canine, Chinese Hamster

Cited:

Canine, Hamster - Cricetulus (Chinese Hamster)

Predicted:

Bovine (100%), Chicken (100%), Human (100%), Mouse (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all MARCKS Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH. Accession # P30009

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID: 17079793). Canine reactivity reported in scientific literature (PMID: 25588777)

Modification

p Ser152, p Ser156

Localization

Cytoplasmic; actin cytoskeleton

Specificity

Specific for endogenous levels of the ~87 kDa MARCKS protein phosphorylated at Ser152 and Ser156. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MARCKS [p Ser152, p Ser156] Antibody - Azide Free

Western Blot: MARCKS [p Ser152, p Ser156] Antibody [NB500-140]

Western Blot: MARCKS [p Ser152, p Ser156] Antibody [NB500-140]

Western Blot: MARCKS [p Ser152, p Ser156] Antibody [NB500-140] - Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Ser152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser 152,156 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for MARCKS [p Ser152, p Ser156] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
NB500-140 can be used in Western blot where a band is seen at ~ 80 kDa, representing MARCKS. Immunohistochemical analysis has not yet been determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MARCKS

Myristolated Alanine-Rich C-Kinase Substrate (MARCKS) is a major in vivo substrate of protein kinase C (PKC) and for the lipid-activated PKC-related kinase (PRK1). Furthermore, PRK1 and OPKC phosphorylate MARCKS on the same sites in vitro, serine 152, 156, and 163. MARCKS serves as an in vitro substrate for PKCu as well. However, in contrast to other PKCs, MARCKS is phosphorylated by PKCu only at serine 156 and not at serines 152 and 163, implicating a differential regulation by PKCu. Therefore, control of MARCKS phosphorylation on these previously identified 'PKC' sites may be regulated under certain circumstances by PRK as well as PKC mediated signaling pathways. MARCKS associates with the plasma membrane in response to PKC-catalyzed phosphorylation of MARCKS. It has been suggested that MARCKS is capable of associating with the plasma membrane through binding to phospholipids without interaction with membranous proteins.

Long Name

Myristoylated Alanine-rich Protein Kinase C Substrate

Alternate Names

80K-L, MACS, PRKCSL

Entrez Gene IDs

4082 (Human); 17118 (Mouse); 25603 (Rat); 396473 (Chicken)

Gene Symbol

MARCKS

UniProt

P30009 (Rat)

Additional MARCKS Products

Product Documents for MARCKS [p Ser152, p Ser156] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for MARCKS [p Ser152, p Ser156] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

Citations for MARCKS [p Ser152, p Ser156] Antibody - Azide Free

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