MARK2 Antibody

Novus Biologicals | Catalog # NBP1-00214

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MARK2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QNGKDSTAPQR corresponding to internal region according to NP_001156768.1, NP_001156769.1.

Specificity

This antibody is expected to recognise isoform c (NP_004945.4), isoform e (NP_001156768.1) and isoform f (NP_001156769.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MARK2 Antibody

Western Blot: MARK2 Antibody [NBP1-00214]

Western Blot: MARK2 Antibody [NBP1-00214]

Western Blot: MARK2 Antibody [NBP1-00214] - (0.3ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MARK2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human brain (cerebellum) lysates (calculated MW of 77.6 kDa band according to NP_004945.3).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MARK2

EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed. [provided by RefSeq]

Alternate Names

EC 2.7.11, EC 2.7.11.1, ELKL motif kinase, ELKL motif kinase 1, EMK-1, EMK1PAR-1, MAP/microtubule affinity-regulating kinase 2MGC99619, PAR1 homolog, Par1b, Ser/Thr protein kinase PAR-1B, serine/threonine protein kinase EMK, serine/threonine-protein kinase MARK2

Entrez Gene IDs

2011 (Human); 13728 (Mouse); 60328 (Rat)

Gene Symbol

MARK2

UniProt

Q7KZI7 (Human)

Additional MARK2 Products

Product Documents for MARK2 Antibody

Certificate of Analysis

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Product Specific Notices for MARK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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