MARK4 Antibody

Novus Biologicals | Catalog # NB100-1013

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry

Cited:

In vitro assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MARK4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence IKRKPPPGCSDSPGV corresponding to C-Terminus according to NP_113605.2.

Specificity

This antibody is expected to recognize the reported long isoform 1 (NP_113605.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MARK4 Antibody

Western Blot: MARK4 Antibody [NB100-1013]

Western Blot: MARK4 Antibody [NB100-1013]

Western Blot: MARK4 Antibody [NB100-1013] - Staining of U251 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: MARK4 Antibody [NB100-1013]

Flow Cytometry: MARK4 Antibody [NB100-1013]

Flow Cytometry: MARK4 Antibody [NB100-1013] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: MARK4 Antibody [NB100-1013]

Western Blot: MARK4 Antibody [NB100-1013]

Western Blot: MARK4 Antibody [NB100-1013] - Staining of Human Cerebellum lysate (A) + peptide (B), Mouse Brain lysate (C) + peptide (D) (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Applications for MARK4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/mL

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MARK4

MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells.

Alternate Names

EC 2.7.11, EC 2.7.11.1, FLJ12177, FLJ90097, KIAA1860MARK4L, MAP/microtubule affinity-regulating kinase 4, MAP/microtubule affinity-regulating kinase like 1, MAP/microtubule affinity-regulating kinase-like 1, MARK4 serine/threonine protein kinase, MARKL1L, MARKL1MARK4S, Nbla00650

Entrez Gene IDs

57787 (Human)

Gene Symbol

MARK4

UniProt

NP_113605. (Human)

Additional MARK4 Products

Product Documents for MARK4 Antibody

Certificate of Analysis

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Product Specific Notices for MARK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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