Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Matriptase/ST14 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 800 and the C-terminus (residue 855) of human Membrane-type serine protease 1 using the numbering given in SwissProt entry Q9Y5Y6 (GeneID 6768).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Matriptase/ST14 Antibody - BSA Free

Western Blot: Matriptase/ST14 Antibody [NB100-2308]

Western Blot: Matriptase/ST14 Antibody [NB100-2308]

Western Blot: Matriptase/ST14 Antibody [NB100-2308] - Detection of Human Matriptase/ST14 by Western Blot. Samples: Whole cell lysate (50 ug) from MCF7, OVCAR4 [OV], SW620 [SW], RPMI8266 [RPMI], and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Matriptase/ST14 antibody NB100-2308 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Matriptase/ST14 Antibody [NB100-2308]

Immunoprecipitation: Matriptase/ST14 Antibody [NB100-2308]

Immunoprecipitation: Matriptase/ST14 Antibody [NB100-2308] - Detection of human Matriptase/ST14 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Matriptase/ST14 antibody NB100-2308 used for IP at 6 ug per reaction. Matriptase/ST14 was also immunoprecipitated by Rabbit anti-Matriptase/ST14 antibodies A, B and C. For blotting immunoprecipitated Matriptase/ST14, NB100-2308 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Matriptase/ST14 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Matriptase/ST14

The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis.

Alternate Names

Epithin, MTSP1, PRSS14, SNC19, ST14, TADG-15, TMPRSS14

Entrez Gene IDs

6768 (Human)

Gene Symbol

ST14

UniProt

Q9Y5Y6

Additional Matriptase/ST14 Products

Product Documents for Matriptase/ST14 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Matriptase/ST14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Matriptase/ST14 Antibody - BSA Free

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