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Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
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Product Specifications
Immunogen
Polyclonal antibody raised in rabbit against mouse MBD2 (methyl-CpG binding domain protein 2), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein (1).
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for MBD2 Antibody
Western Blot: MBD2 Antibody [NBP3-18666]
Western Blot: MBD2 Antibody [NBP3-18666] - Figure 2. Western blot analysis using the antibody directed against MBD2 (1) Western blot was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse MBD2, diluted 1:500 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right.ELISA: MBD2 Antibody [NBP3-18666]
ELISA: MBD2 Antibody [NBP3-18666] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse MBD2. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:185,000.Applications for MBD2 Antibody
Application
Recommended Usage
ELISA
1:1000-1:4000
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Whole Antiserum
Formulation
Whole antiserum
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: MBD2
Long Name
Methyl-CpG-binding domain protein 2
Alternate Names
Demethylase, DMTase
Gene Symbol
MBD2
UniProt
Additional MBD2 Products
Product Documents for MBD2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MBD2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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