MBD2 Antibody

Novus Biologicals | Catalog # NBP3-18666

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
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Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against mouse MBD2 (methyl-CpG binding domain protein 2), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein (1).

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for MBD2 Antibody

Western Blot: MBD2 Antibody [NBP3-18666]

Western Blot: MBD2 Antibody [NBP3-18666]

Western Blot: MBD2 Antibody [NBP3-18666] - Figure 2. Western blot analysis using the antibody directed against MBD2 (1) Western blot was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse MBD2, diluted 1:500 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
ELISA: MBD2 Antibody [NBP3-18666]

ELISA: MBD2 Antibody [NBP3-18666]

ELISA: MBD2 Antibody [NBP3-18666] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse MBD2. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:185,000.

Applications for MBD2 Antibody

Application
Recommended Usage

ELISA

1:1000-1:4000

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: MBD2

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. [provided by RefSeq]

Long Name

Methyl-CpG-binding domain protein 2

Alternate Names

Demethylase, DMTase

Gene Symbol

MBD2

UniProt

Additional MBD2 Products

Product Documents for MBD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MBD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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