MBL1 Antibody (8G6)

Novus Biologicals | Catalog # NBP1-60127

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoassay, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 8G6
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Product Specifications

Immunogen

mouse MBL-A

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for MBL1 Antibody (8G6)

Immunohistochemistry-Frozen: MBL1 Antibody (8G6) [NBP1-60127]

Immunohistochemistry-Frozen: MBL1 Antibody (8G6) [NBP1-60127]

Immunohistochemistry-Frozen: MBL1 Antibody (8G6) [NBP1-60127] - MBL-A (8G6) deposition in developing murine atherosclerotic lesions. Staining of frozen tissue sections with antibody 8G6. Anti-mouse MBL-A at 2ug/ml (2h, RT). MBL-A was detected on the intima to media border as well as throughout the media (insert). Furthermore, extensive MBL-A deposition was seen at sites of necrosis (upper right corner).

Applications for MBL1 Antibody (8G6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

2 ug/ml

Western Blot

1:100-1:2000
Application Notes
In Western blots, non-reduced sample treatment was used. The band sizes were 191, 263 and 316 kDa (see Liu, H et al.). For IHC-F: Tissue sections were fixed in 4% PBS-buffered formaldehyde and pretreated with 2% hydrogen peroxide in methanol for 20 minutes at 4 degrees Celsius to quench endogenous peroxidases. (See Windbichler, M et al and Matthijsen, R et al)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MBL-1

Mannose Binding Lectin (MBL), also called mannosebinding protein (MBP), is a calcium dependent oligomeric protein that belongs to the collectin family of proteins. It contains a collagen-like domain and a carbohydrate recognition domain enabling MBL to recognize carbohydrates (such as mannose and N-acetylglucosamine) on pathogens. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways, by using attached mannose binding lectin-associated serine proteases (MASP-2) in an antibody- and C1q-independent manner. MASP-2 permits cleavage of C4 and C2 to form a C3 convertase. Once it has bound, MBL exhibits complement-dependent antibacterial activities such as microbial opsonization and/or microbial lysis via membrane attack complexes and therefore plays an important role in innate immunity. In human, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, termed MBL-A and MBL-C. The MBL-A concentration in serum is about 6-fold lower compared to that of MBL-C... MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. Moreover, it has been shown that MBL-A deficient mice have aberrant antigen-specific IgM responses and suffer from increased susceptibility to infection. Note that the monoclonal antibody 8G6 is a calcium-dependent antibody.

Long Name

Mannose Binding Lectin

Alternate Names

MBL-A, MBL1, MBP-A, S-MBP

Gene Symbol

Mbl1

Additional MBL-1 Products

Product Documents for MBL1 Antibody (8G6)

Certificate of Analysis

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Product Specific Notices for MBL1 Antibody (8G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MBL1 Antibody (8G6)

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