MCM4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85728

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MCM4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSAT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM4 Antibody - BSA Free

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Analysis in human testis and pancreas tissues. Corresponding MCM4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human pancreas shows low positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728]

Immunohistochemistry-Paraffin: MCM4 Antibody [NBP1-85728] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.
MCM4 Antibody - BSA Free Western Blot: MCM4 Antibody - BSA Free [NBP1-85728]

Western Blot: MCM4 Antibody - BSA Free [NBP1-85728]

Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MCM4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
MCM4 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: MCM4 Antibody - BSA Free [NBP1-85728]

Immunocytochemistry/ Immunofluorescence: MCM4 Antibody - BSA Free [NBP1-85728]

Staining of human cell line U-2 OS shows localization to nucleoplasm.

Applications for MCM4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM4

MCM4 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Long Name

minichromosome maintenance complex component 4

Alternate Names

CDC21, CDC54, hCdc21, IMD54, NKCD, NKGCD, P1-CDC21

Gene Symbol

MCM4

Additional MCM4 Products

Product Documents for MCM4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MCM4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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