MCM6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82642

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation Sequencing, Knockdown Validated

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MCM6 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VILRAEAVESAQAGDKCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLACCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNLCTSLFPTIHGNDEVKRGV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM6 Antibody - BSA Free

Western Blot: MCM6 Antibody [NBP1-82642]

Western Blot: MCM6 Antibody [NBP1-82642]

Western Blot: MCM6 Antibody [NBP1-82642] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: MCM6 Antibody [NBP1-82642]

Immunocytochemistry/ Immunofluorescence: MCM6 Antibody [NBP1-82642]

Immunocytochemistry/Immunofluorescence: MCM6 Antibody [NBP1-82642] - Staining of human cell line U-2 OS shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642]

Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642]

Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642] - Staining in human lymph node and liver tissues using anti-MCM6 antibody. Corresponding MCM6 RNA-seq data are presented for the same tissues.
Western Blot: MCM6 Antibody [NBP1-82642]

Western Blot: MCM6 Antibody [NBP1-82642]

Western Blot: MCM6 Antibody [NBP1-82642] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MCM6 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642]

Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642]

Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642]

Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642]

Immunohistochemistry-Paraffin: MCM6 Antibody [NBP1-82642] - Staining of human lymph node shows high expression.
MCM6 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: MCM6 Antibody - BSA Free [NBP1-82642]

Chromatin Immunoprecipitation-exo-Seq: MCM6 Antibody - BSA Free [NBP1-82642]

ChIP-Exo-Seq composite graph for Anti-MCM6 (NBP1-82642) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for MCM6 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation Sequencing

1-10µg per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM6

MCM6 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.

Alternate Names

DNA replication licensing factor MCM6, EC 3.6.4.12, MCG40308, MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe), MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S.cerevisiae), minichromosome maintenance complex component 6, minichromosome maintenance deficient (mis5, S. pombe) 6, minichromosome maintenance deficient 6 homolog, minichromosome maintenance deficient 6 homolog (S. cerevisiae), Mis5, MIS5 homolog, p105MCM

Gene Symbol

MCM6

Additional MCM6 Products

Product Documents for MCM6 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MCM6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MCM6 Antibody - BSA Free

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Protocols

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