MEF2D Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85795

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MEF2D Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VPVSNQSSLQFSNPSGSLVTPSLVTSSLTDPRLLSPQQPALQRNSVSPGLPQRPASAGAMLGGDLNSANGACPSPVGNGYVSARASPGLLPVANGNSLNKVIPAKSPPPPTHSTQLGAPSRKPDLRVITSQAG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEF2D Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MEF2D Antibody [NBP1-85795]

Immunocytochemistry/ Immunofluorescence: MEF2D Antibody [NBP1-85795]

Immunocytochemistry/Immunofluorescence: MEF2D Antibody [NBP1-85795] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Analysis in human skeletal muscle and liver tissues. Corresponding MEF2D RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Staining of human kidney, liver, placenta and skeletal muscle using Anti-MEF2D antibody NBP1-85795 (A) shows similar protein distribution across tissues to independent antibody NBP1-85788 (B).
Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
MEF2D Antibody - BSA Free Immunohistochemistry-Paraffin: MEF2D Antibody - BSA Free [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody - BSA Free [NBP1-85795]

Staining of human skeletal muscle shows strong nuclear positivity in myocytes.
MEF2D Antibody - BSA Free Immunohistochemistry-Paraffin: MEF2D Antibody - BSA Free [NBP1-85795]

Immunohistochemistry-Paraffin: MEF2D Antibody - BSA Free [NBP1-85795]

Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
MEF2D Antibody - BSA Free Western Blot: MEF2D Antibody - BSA Free [NBP1-85795]

Western Blot: MEF2D Antibody - BSA Free [NBP1-85795]

Analysis in control (vector only transfected HEK293T lysate) and MEF2D over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for MEF2D Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEF2D

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis

Alternate Names

DKFZp686I1536, MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancerfactor 2D), MEF2D/DAZAP1 fusion, myocyte enhancer factor 2D, myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusionprotein, myocyte-specific enhancer factor 2D

Gene Symbol

MEF2D

Additional MEF2D Products

Product Documents for MEF2D Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MEF2D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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