MEK1/2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05651

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MEK1/MEK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MAP2K1 (NP_002746.1). SRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEK1/2 Antibody - BSA Free

Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]

Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]

Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of various cell lines, using MAP2K1/MAP2K2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.
Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]

Western Blot: MEK1/2 AntibodyBSA Free [NBP3-05651]

Western Blot: MEK1/2 Antibody [NBP3-05651] - Analysis of extracts of PC-12 cells, using MEK1/2 antibody (NBP3-05651) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for MEK1/2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK1/MEK2

MEK1 and MEK2, also known as MAP2K1 and MAP2K2, are activated in response to mitogenic stimuli. Activation occurs through dual phosphorylation at S218/S222 of MEK1 and S222/S226 of MEK2 by several upstream MAP3Ks. Both MEK1 and MEK2 are dual-specificity protein kinases, phosphorylating and activating ERK1 and ERK2 at Thr and Tyr positions.

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

CFC3, CFC4, dual specificity mitogen-activated protein kinase kinase 1, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK 1, MAPKK1, MAPKK2, MEK 1, MEK1, MEK2, MEL, mitogen-activated protein kinase kinase 2, p45, MKK1, MKK2, PRKMK1, PRKMK2, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Gene Symbol

MAP2K1

Additional MEK1/MEK2 Products

Product Documents for MEK1/2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MEK1/2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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