Melanocortin-1 R/MC1R Antibody - BSA Free

Novus Biologicals | Catalog # NLS1040

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Predicted:

Chimpanzee (100%), Orangutan (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Melanocortin-1 R/MC1R Primary Antibodies »

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Melanocortin-1 R/MC1R.

Epitope

3rd cytoplasmic domain of human

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%).

Specificity

Human Melanocortin-1 R/MC1R. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Melanocortin-1 R/MC1R Antibody - BSA Free

Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody - BSA Free [NLS1040]

Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody - BSA Free [NLS1040]

Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody - BSA Free [NLS1040]

Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody - BSA Free [NLS1040]

Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Applications for Melanocortin-1 R/MC1R Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 - 5 ug/ml
Application Notes
.

Reviewed Applications

Read 1 review rated 5 using NLS1040 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Melanocortin-1 R/MC1R

MC1R, a Melanocortin Receptor, mediates the effects of melanocyte-stimulating hormone (MSH) and adrenocorticotropic hormone (ACTH). MSH and its receptor, MC1R, are key regulators of melanogenesis. They control the relative proportions of red pheomelanin and black photoprotective eumelanin in the skin by increasing the production of eumelanin. Non-functional, truncated forms of the receptor lead to lighter coat color in animals. In contrast, constitutively active receptors lead to darker color. Pheomelanin generates free radicals in response to UV radiation, and elevated levels of pheomelanin have been associated with higher risk of UV-induced skin damage. Therefore, MC1R is a major determinant of sun sensitivity and genetic risk for melanoma and other skin cancers. Expression of melanocortin 1 receptor has been reported primarily in adrenal, skin, and testis. ESTs have been isolated from breast, placenta, and testis libraries.

Long Name

Melanocortin 1 Receptor

Alternate Names

CMM5, MC1R, Melanocortin-1R, Melanocortin1R, MSH-R, Mshra, SHEP2, Tob

Entrez Gene IDs

4157 (Human)

Gene Symbol

MC1R

Additional Melanocortin-1 R/MC1R Products

Product Documents for Melanocortin-1 R/MC1R Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Melanocortin-1 R/MC1R Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Melanocortin-1 R/MC1R Antibody - BSA Free (1)

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  • Melanocortin-1 R/MC1R Antibody
    Name: Quentin Howlett-Prieto
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 02/05/2019
    Figure 1 shows Lymphocytes stained with Alexa Fluor 488 Donkey anti-rabbit IgG alone, and Figure 2 shows Lymphocytes stained with human MC1R and Alexa Fluor 488 Donkey anti-rabbit IgG.
    Blocking: Natural Goat Serum Primary antibody: 2 uL (1 ug) Secondary antibody: Alexa Fluor 488 Donkey anti-rabbit IgG (2.5 uL / 1 ug) Details: Human lymphocytes were isolated from PBMCs. Lymphocytes were blocked with Natural Goat Serum for 15 minutes, and then stained and subsequently incubated at -20 C with anti-human MC1R primary antibody for 30 minutes. After washing, cells were stained with Alexa Fluor 488 Donkey anti-rabbit IgG and incubated at -20 C for 40 minutes. After washing, cells were detected using a Fortessa 4-15 flow cytometer.
    Melanocortin-1 R/MC1R Antibody - BSA Free NLS1040

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Protocols

View specific protocols for Melanocortin-1 R/MC1R Antibody - BSA Free (NLS1040):

Immunohistochemistry Protocol for MC1 Receptor Antibody (NLS1040):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4-um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene approximately 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol approximately 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol approximately 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol approximately 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water approximately 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block approximately 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody approximately 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T approximately 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary approximately 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
17. Apply an alkaline phosphatase steptavidin approximately 30 minutes at RT.
18. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
19. Apply an alkaline phosphatase chromagen substrate approximately 30 minutes at RT.
20. Rinse the slide in distilled water approximately 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol approximately 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol approximately 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol approximately 1 minute each @ RT.
24. Wash the slides in 3 changes of xyleneapproximately 1 minute each @ RT.
25. Apply cover slip.

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