Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)
Novus Biologicals | Catalog # NBP2-34341
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa/IgG1 Kappa Clone # DT101+ BC199 + HMB45
Loading...
Product Specifications
Immunogen
Recombinant Melanoma Marker (MART-1 + gp100) protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa/IgG1 Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34608)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)
![Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]](https://resources.rndsystems.com/images/products/Melanoma-Marker-MART-1-+-gp100-Antibody-DT101+-BC199-+-HMB45-Immunohistochemistry-Paraffin-NBP2-34341-img0005.jpg "Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]")
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341] - Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45).![Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]](https://resources.rndsystems.com/images/products/Melanoma-Marker-MART-1-+-gp100-Antibody-DT101+-BC199-+-HMB45-Immunohistochemistry-Paraffin-NBP2-34341-img0004.jpg "Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]")
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341]
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [NBP2-34341] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (DT101+ BC199 + HMB45).Applications for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Flow Cytometry reported in scientific literature (PMID: 30735560).
Optimal dilution for a specific application should be determined.
Use in Flow Cytometry reported in scientific literature (PMID: 30735560).
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Melanoma Marker (MART-1 + gp100)
Additional Melanoma Marker (MART-1 + gp100) Products
Product Documents for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)
Powered by Bioz
Customer Reviews for Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)
There are currently no reviews for this product. Be the first to review Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) and earn rewards!
Have you used Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...