Mena Antibody - BSA Free

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human fallopian tube, liver, prostate and testis using Anti-ENAH antibody (A) NBP1-87914 shows similar protein distribution across tissues to independent antibody NBP1-87915 (B).

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human Fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human liver shows low positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human prostate shows moderate to strong cytoplasmic and membranous positivity in glandular cells and smooth muscle cells.

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87914] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Western Blot: Mena Antibody - BSA Free [NBP1-87914]

Analysis in human cell lines U2OS and MCF-7 using Anti-ENAH antibody. Corresponding ENAH RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Western Blot: Mena Antibody - BSA Free [NBP1-87914]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/ Immunofluorescence: Mena Antibody - BSA Free [NBP1-87914] -

MAL2 overexpression disrupts invadopodia formation. (A) Lysates from uninfected Clone 9 cells and Clone 9 cells expressing wild type (WT) MAL2 were immunoblotted for Tks5, Mena, VASP, FAK and tubulin (as a loading control) as indicated. Molecular weight standards are indicated on the left in kDa. (B) Uninfected Clone 9 cells and Clone 9 cells expressing wild type MAL2 were immunolabeled for Tks5 or Mena as indicated. Arrowheads in panels a and b mark Tks5 and Mena present in juxta-nuclear invadopodia that are absent in cells expressing MAL2. (C) Uninfected and MAL2-expressing cells were seeded onto 96-well plates at 4000 cells/well and relative cell numbers monitored after 1 (p ≤ 0.006), 2 (p ≤ 0.006) or 3 days. Values represent the mean +/- SEM from at least three independent experiments each performed in triplicate. (D) Uninfected Clone 9 cells were labeled for Mena. Arrowheads mark Mena present at the surface in patches. (E) Clone 9 cells expressing wild type MAL2 were immunolabeled for Mena and MAL2 as indicated. Arrowheads mark Mena present at the far distal tips of protrusions induced by MAL2 expression. Bar = 10 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32059473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.