Mena Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87915

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: STSTPEPTRKPWERTNTMNGSKSPVISRRDSPRKNQIVFDNRSYDSLHRPKSTPLSQPSANGVQTEGLDYDRLKQDILDEMRKELTKLKEELIDAIRQELSKSN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mena Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Mena Antibody [NBP1-87915]

Immunocytochemistry/ Immunofluorescence: Mena Antibody [NBP1-87915]

Immunocytochemistry/Immunofluorescence: Mena Antibody [NBP1-87915] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Analysis in human testis and liver tissues. Corresponding ENAH RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human endometrium, liver, pancreas and tonsil using Anti-AGXT antibody (A) NBP1-87915 shows similar protein distribution across tissues to independent antibody NBP1-87914 (B).
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human prostate shows moderate to strong cytoplasmic and membranous positivity in glandular cells and smooth muscle cells.
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human Fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human liver shows low positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915]

Immunohistochemistry-Paraffin: Mena Antibody [NBP1-87915] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Mena Antibody - BSA Free Western Blot: Mena Antibody - BSA Free [NBP1-87915]

Western Blot: Mena Antibody - BSA Free [NBP1-87915]

Analysis in human cell lines U2OS and MCF-7 using Anti-ENAH antibody. Corresponding ENAH RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Applications for Mena Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mena

ENAH/MENA belongs to the Ena/vasodilator-stimulated phosphoprotein (VASP) family of proteins which play a role in regulating the actin cytoskeleton for the control of cell shape and movement. The Ena/VASP family of actin binding proteins includes ENAH/MENA which is the human homolog of drosophila Ena (enabled), VASP, and EVL (Ena/vasodilator-stimulated phosphoprotein-like protein). Ena/VASP proteins contain an EVH1 domain, a central proline-rich domain, and a terminal EVH2 domain. ENAH/MENA is not expressed in normal breast but has been detected and associated with breast lesions with a high risk of transformation and primary and metastatic breast cancer.

Alternate Names

ENAH, NDPP1

Gene Symbol

ENAH

Additional Mena Products

Product Documents for Mena Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Mena Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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