MEPE/OF45 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-16826

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all MEPE/OF45 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 90-256 of human MEPE/OF45 (NP_064588.1). FTNRQRLNKEYSISNKENTHNGLRMSIYPKSTGNKGFEDGDDAISKLHDQEEYGAALIRNNMQHIMGPVTAIKLLGEENKENTPRNVLNIIPASMNYAKAHSKDKKKPQRDSQAQKSPVKSKSTHRIQHNIDYLKHLSKVKKIPSDFEGSGYTDLQERGDNDISPFS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEPE/OF45 Antibody - Azide and BSA Free

Western Blot: MEPE/OF45 AntibodyAzide and BSA Free [NBP3-16826]

Western Blot: MEPE/OF45 AntibodyAzide and BSA Free [NBP3-16826]

Western Blot: MEPE/OF45 Antibody [NBP3-16826] - Western blot analysis of extracts of various cell lines, using MEPE/OF45 antibody (NBP3-16826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for MEPE/OF45 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEPE/OF45

MEPE, known as OF45 in mouse and rat, is a 55 kDa member of the SIBLING protein family that is primarily expressed in bone and dentin where it regulates the mineralization of those tissues. MEPE is secreted by osteoblasts and dental pulp stem cells during the mineralization process and also by non-mineralizing tissues including epithelial cells in the renal proximal tubule and salivary duct. MEPE contains multiple consensus sites for post-translational modifications, including N-linked glycosylation, N-myristoylation, glycosaminoglycan attachment, and phosphorylation by a variety of kinases. MEPE also contains several putative proteolytic cleavage sites and one integrin-binding RGD motif.

Long Name

Matrix Extracellular Phosphoglycoprotein with ASARM Motif

Alternate Names

OF45, Osteoregulin

Gene Symbol

MEPE

Additional MEPE/OF45 Products

Product Documents for MEPE/OF45 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MEPE/OF45 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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