MESDC2 Antibody (502) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89719

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 502 expressed in HEK293

Format

Azide and BSA Free
View all MESDC2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human MESDC2 (Accession#: NP_055969.1; Ala 34-Lys 230).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MESDC2 Antibody (502) - Azide and BSA Free

Western Blot: MESDC2 Antibody (502) [NBP2-89719]

Western Blot: MESDC2 Antibody (502) [NBP2-89719]

Western Blot: MESDC2 Antibody (502) [NBP2-89719] - Lane A: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:26 kDa
Immunoprecipitation: MESDC2 Antibody (502) [NBP2-89719]

Immunoprecipitation: MESDC2 Antibody (502) [NBP2-89719]

Immunoprecipitation: MESDC2 Antibody (502) [NBP2-89719] - Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg NIH-3T3 Whole Cell Lysate 2 uL anti-MESDC2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-MESDC2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size: 26 kDa
MESDC2 Antibody (502) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: MESDC2 Antibody (502) [NBP2-89719] -

Immunocytochemistry/Immunofluorescence: MESDC2 Antibody (502) [NBP2-89719] - Staining of Mesdc2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89719 antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.

Applications for MESDC2 Antibody (502) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

1-4 ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MESDC2

Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway, since some LDLRs are coreceptors for the canonical Wnt pathway. Essential for specification of embryonic polarity and mesoderm induction

Long Name

Mesoderm Development Candidate 2

Alternate Names

BOCA

Gene Symbol

MESD

Additional MESDC2 Products

Product Documents for MESDC2 Antibody (502) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MESDC2 Antibody (502) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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