Mesothelin Antibody (SPM143)

Novus Biologicals | Catalog # NBP3-09172

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SPM143
View all Mesothelin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary) (Uniprot: Q13421)

Localization

Cell Surface and Secreted

Marker

Mesothelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11529)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Mesothelin Antibody (SPM143)

Immunohistochemistry-Paraffin: Mesothelin Antibody (SPM143) [NBP3-09172]

Immunohistochemistry-Paraffin: Mesothelin Antibody (SPM143) [NBP3-09172]

Immunohistochemistry-Paraffin: Mesothelin Antibody (SPM143) [NBP3-09172] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Mesothelin Mouse Monoclonal Antibody (SPM143).
Immunohistochemistry-Paraffin: Mesothelin Antibody (SPM143) [NBP3-09172]

Immunohistochemistry-Paraffin: Mesothelin Antibody (SPM143) [NBP3-09172]

Immunohistochemistry-Paraffin: Mesothelin Antibody (SPM143) [NBP3-09172] - Formalin-fixed, paraffin-embedded human Lung Mesothelioma stained with Mesothelin Mouse Monoclonal Antibody (SPM143).

Applications for Mesothelin Antibody (SPM143)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Mesothelin

MSLN, also known as Mesothelin, has 4 isoforms, a 630 amino acid isoform that is 69 kDa, a 622 amino acid isoform that is 68 kDa, a 656 amino acid isoform that is 71 kDa, and a 621 amino acid isoform that is 68 kDa, expressed in lung, heart, placenta kidney, and overexpressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas. Membrane-anchored forms may play a role in cellular adhesion and megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Current research is being performed on several diseases and disorders including squamous cell carcinoma, ovarian cancer, carcinoma, malignant biphasic mesothelioma, bile duct adenoma, malignant mesothelioma, malignant pleural mesothelioma, gastrointestinal stromal tumor, renal clear cell carcinoma, epithelioid sarcoma, benign mesothelioma, pancreatic ductal adenocarcinoma, asbestosis, pancreatic ductal carcinoma, thymic carcinoma, acute myeloid leukemia, epithelial ovarian cancer, myeloid leukemia, thoracic cancer, and pancreatitis. This protein has also shown an interaction with MUC16 and RSG1 in cell adhesion and pancreas development processes and pathways.

Alternate Names

CAK1, MPF, MSLN, SMR

Gene Symbol

MSLN

UniProt

Q13421

Additional Mesothelin Products

Product Documents for Mesothelin Antibody (SPM143)

Certificate of Analysis

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Product Specific Notices for Mesothelin Antibody (SPM143)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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