metallothionein Antibody (N11-G)

Novus Biologicals | Catalog # NBP1-79083

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rabbit

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # N11-G
View all metallothionein Primary Antibodies »

Product Specifications

Immunogen

Peptide corresponding to the N-terminal sequence of human metallothionein (covering isoforms 1A, B, E, F, G, H, I, L, M, Q, S, Y, 2A)

Reactivity Notes

Human - isoforms: 1A, 1B, 1E, 1F, 1G,1H, 1I,1L, Rabbit - isoforms: 1A, 2A

Specificity

NBP1-79083 immunogen is identical and covers isoforms#: 1A P04731, #1B P07438, #1E P04732,#1F P04733, #1G P13640, #1H P80294, #1I P80295, #1L Q93083, #1M Q8N339, #1Q Q86YX0, #1S Q86YX5, #1Y Q8TDC4, #2A P18055.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for metallothionein Antibody (N11-G)

Western Blot: metallothionein Antibody (N11-G) [NBP1-79083]

Western Blot: metallothionein Antibody (N11-G) [NBP1-79083]

Western Blot: metallothionein Antibody (N11-G) [NBP1-79083] - Rabbit liver. Lane 1 - 1ug; Lane 2 - 3ug; Lane 3 - 5ug of protein loaded.

Applications for metallothionein Antibody (N11-G)

Application
Recommended Usage

ELISA

1:5000-1:10000

Western Blot

1:1000
Application Notes
For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: metallothionein

Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.

Additional metallothionein Products

Product Documents for metallothionein Antibody (N11-G)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for metallothionein Antibody (N11-G)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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