Methyl-Lysine Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-78104

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Methyl-Lysine Primary Antibodies »

Product Specifications

Immunogen

This affinity-purified Methyl-Lysine Antibody was prepared from whole rabbit serum produced by repeated immunizations with Methyl-Lysine conjugated KLH.

Reactivity Notes

The cross reactivity with tri-methyl lysine has not been tested. There are no cross reactions with acetylated proteins

Specificity

Reactivity is specific for Methylated Lysine residues (mono, di).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Applications for Methyl-Lysine Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000-1:20000

Western Blot

1:500-1:3000
Application Notes
This affinity-purified antibody is suitable for use in ELISA, immunohistochemistry, western blotting and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, and flow cytometry.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0, 50% (v/v) Glycerol

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Methyl-Lysine

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

Alternate Names

Dimethylysine, Methyl lysine, methyllysine, monomethyl lysine, N epsilon dimethyl lysine, N epsilon mono methyllysine, N epsilon trimethyl lysine, N(epsilon) trimethyl lysine, Trimethyl lysine

Additional Methyl-Lysine Products

Product Documents for Methyl-Lysine Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Methyl-Lysine Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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