Methyl-Lysine Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-15955

Novus Biologicals
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Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, ELISA, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Methyl-Lysine Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence containing Tri-methylated K.

Reactivity Notes

Wide Range Predicted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methyl-Lysine Antibody - Azide and BSA Free

Western Blot: Methyl-Lysine AntibodyAzide and BSA Free [NBP3-15955]

Western Blot: Methyl-Lysine AntibodyAzide and BSA Free [NBP3-15955]

Western Blot: Methyl-Lysine Antibody [NBP3-15955] - Analysis of extracts of various cell lines, using Methyl-Lysine antibody (NBP3-15955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunoprecipitation: Methyl-Lysine Antibody - Azide and BSA Free [NBP3-15955]

Immunoprecipitation: Methyl-Lysine Antibody - Azide and BSA Free [NBP3-15955]

Immunoprecipitation: Methyl-Lysine Antibody [NBP3-15955] - Analysis of 1.5mg extracts of HeLa cells using 30ug Methyl-Lysine antibody (NBP3-15955). Western blot was performed from the immunoprecipitate using TriMethyl-Histone H3-K27 antibody (A2363) at a dilition of 1:1000.
Methyl-Lysine Antibody - Azide and BSA Free

Chromatin Immunoprecipitation: Methyl-Lysine Antibody - Azide and BSA Free [Methyl-Lysine] -

Chromatin Immunoprecipitation: Methyl-Lysine Antibody - Azide and BSA Free [Methyl-Lysine] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Methyl-Lysine antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Applications for Methyl-Lysine Antibody - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

3μg antibody for 5μg-10μg of Chromatin

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunoprecipitation

5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Methyl-Lysine

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

Alternate Names

Dimethylysine, Methyl lysine, methyllysine, monomethyl lysine, N epsilon dimethyl lysine, N epsilon mono methyllysine, N epsilon trimethyl lysine, N(epsilon) trimethyl lysine, Trimethyl lysine

Additional Methyl-Lysine Products

Product Documents for Methyl-Lysine Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Methyl-Lysine Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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