Methyl-Lysine Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35817

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Methyl-Lysine Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence containing monomethylated K.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methyl-Lysine Antibody - BSA Free

Methyl-Lysine Antibody

Western Blot: Methyl-Lysine Antibody [NBP3-35817] -

Western Blot: Methyl-Lysine Antibody [NBP3-35817] - Western blot analysis of various lysates using Methyl-Lysine Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Methyl-Lysine Antibody

Western Blot: Methyl-Lysine Antibody [NBP3-35817] -

Western Blot: Methyl-Lysine Antibody [NBP3-35817] - Western blot analysis of various lysates using Methyl-Lysine Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Methyl-Lysine Antibody

Western Blot: Methyl-Lysine Antibody [NBP3-35817] -

Western Blot: Methyl-Lysine Antibody [NBP3-35817] - Western blot analysis of various lysates using Methyl-Lysine Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.

Applications for Methyl-Lysine Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Methyl-Lysine

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

Alternate Names

Dimethylysine, Methyl lysine, methyllysine, monomethyl lysine, N epsilon dimethyl lysine, N epsilon mono methyllysine, N epsilon trimethyl lysine, N(epsilon) trimethyl lysine, Trimethyl lysine

Additional Methyl-Lysine Products

Product Documents for Methyl-Lysine Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Methyl-Lysine Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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