METTL1 Antibody (9C2F2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-06651

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA Detection, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9C2F2

Format

Azide and BSA Free
View all METTL1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human METTL1 (Accession#: NP_005362.3; Asp 32-Gln 265).

Reactivity Notes

No cross-reactivity in ELISA with:

E.coli cell lysate

Specificity

No cross-reactivity in ELISA with: E.coli cell lysate

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for METTL1 Antibody (9C2F2) - Azide and BSA Free

Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]

Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]

Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651] - NBP3-06651 at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique and performed under reducing conditions. A band appears at an observed molecular weight of 31 kDa.
Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]

Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651]

Western Blot: METTL1 Antibody (9C2F2) [NBP3-06651] - METTL1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. 2 uL NBP3-06651 and 15 ul of 50 % Protein G agarose.Primary antibody: NBP3-06651 at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique and performed under reducing conditions. A band appears at an observed molecular weight of 34 kDa.

Applications for METTL1 Antibody (9C2F2) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:1000-1:2000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000
Application Notes
This antibody will detect Human METTL1 in ELISA pair set (Catalog: # NBP2-79646). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP3-06375).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: METTL1

METTL1 is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq]

Alternate Names

C12orf1, D1075-like gene product, EC 2.1.1.33, FLJ95748, methyltransferase like 1, methyltransferase-like 1, Methyltransferase-like protein 1, TRM8, tRNA (guanine-N(7)-)-methyltransferase, tRNA(m7G46)-methyltransferase, YDL201w

Gene Symbol

METTL1

Additional METTL1 Products

Product Documents for METTL1 Antibody (9C2F2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for METTL1 Antibody (9C2F2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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