METTL14 Antibody (CL4252) - BSA Free

Novus Biologicals | Catalog # NBP2-59043

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL4252

Format

BSA Free
View all METTL14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9HCE5, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for METTL14 Antibody (CL4252) - BSA Free

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043] - Staining in human testis and skeletal muscle tissues. Corresponding METTL14 RNA-seq data are presented for the same tissues.
Western Blot: METTL14 Antibody (CL4252) [NBP2-59043]

Western Blot: METTL14 Antibody (CL4252) [NBP2-59043]

Western Blot: METTL14 Antibody (CL4252) [NBP2-59043] - Analysis in human cell line A-431.
Immunocytochemistry/ Immunofluorescence: METTL14 Antibody (CL4252) [NBP2-59043]

Immunocytochemistry/ Immunofluorescence: METTL14 Antibody (CL4252) [NBP2-59043]

Immunocytochemistry/Immunofluorescence: METTL14 Antibody (CL4252) [NBP2-59043] - Staining of RH-30 cells using the Anti-METTL14 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.
Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043] - Staining of human duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043]

Immunohistochemistry-Paraffin: METTL14 Antibody (CL4252) [NBP2-59043] - Staining of human skeletal muscle shows only weak nuclear positivity in striated muscle fibers.

Applications for METTL14 Antibody (CL4252) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: METTL14

Probable methyltransferase

Long Name

Methyltransferase-Like Protein 14

Alternate Names

KIAA1627

Gene Symbol

METTL14

Additional METTL14 Products

Product Documents for METTL14 Antibody (CL4252) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for METTL14 Antibody (CL4252) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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